Single cell genomics
Background
Genomic analysis is usually performed on millions of cells at a time. This has been necessary in the past in order to extract enough DNA, proteins, and other molecules for analysis, but is problematic when the sample contains a mixture of different cell types.
Recent studies, such as cancer stem cell research, have highlighted the importance of identifying and characterizing rare cell types in mixed populations. We therefore need to develop new techniques that will allow us to analyse the genomes of single cells.
Aims and Relevance
Single cell genomic analysis requires highly specialized equipment that can handle tiny volumes of liquid. This project aims to develop equipment and techniques to capture single cells, and to extract and analyse their DNA. The initial phase will use a variety of cell types, including human mammary cells, to help optimize the new techniques.